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Genome Engineering

All articles tagged with #genome engineering

science-and-technology1 year ago

"Genome Engineering Tool: Beyond CRISPR"

Belgian researchers have developed a new toolbox of 16 short DNA sequences that enable controlled and specific recombination events in any genome, complementing and surpassing CRISPR for certain applications. This toolbox, reported in two concurrent papers in Nature Communications, utilizes site-specific recombinases to address the shortcomings of CRISPR, offering a solution to issues such as toxicity of DNA double strand breaks, low editing efficiencies, and complex patenting. The toolbox has been demonstrated to efficiently optimize gene expression in microbial cell factories and offers possibilities for scientists and industry working in genome engineering.

via Phys.org|
#crispr#genetic-engineering#genome-engineering
science-and-technology2 years ago

"Regulating Genome Engineering: The Role of Anti-CRISPR Proteins"

Scientists have developed off-switches for the Type I-C/Cas3 gene editor, a newer CRISPR platform with potential therapeutic use, to improve its safety. These off-switches, derived from anti-CRISPR proteins that bacteriophages have evolved to combat bacterial CRISPR immunity, can help mitigate off-target effects and increase the safety profile of CRISPR-based genome editing. The study, published in Molecular Cell, provides insights into the mechanisms of these off-switches, offering a potential path towards safer genome engineering.

via Phys.org|
#crispr#genetic-editing#genome-engineering
science-and-technology2 years ago

"pAblo·pCasso: Revolutionizing CRISPR for Advanced Genome Engineering"

Researchers at The Novo Nordisk Foundation Center for Biosustainability have developed a new CRISPR-Cas toolkit called "pAblo·pCasso," which enables precise and reversible DNA edits within Gram-negative bacteria, expanding the range of genome sites available for base-editing and accelerating the development of bacteria for various bioproduction applications. This technology overcomes limitations of traditional CRISPR systems by not requiring specific PAM sequences and offering reversible editing capability, revolutionizing bacterial engineering and genetic manipulation.

via Phys.org|
#bacterial-engineering#crispr#genetic-manipulation
science-and-technology2 years ago

"Breakthrough: Engineered Yeast Achieves Over 50% Synthetic DNA in Groundbreaking Project"

Biologists have successfully engineered a strain of yeast with a genome that is over 50% synthetic DNA. The achievement was made by editing and synthesizing 6.5 of the yeast's chromosomes in the laboratory, along with stitching together an additional chromosome from edited bits of the genetic code. The project, led by the Sc2.0 consortium, aims to create a strain of yeast with a fully synthetic genome, which would be the first eukaryote with such a genome. The engineered yeast has potential applications in producing drugs and fuels, and the project is also advancing biological engineering methods.

via Nature.com|
#biotechnology#genetic-modification#genome-engineering
science-and-technology2 years ago

Unraveling CRISPR's Molecular Scissors: A Genesis Exploration

Researchers have traced the origins of CRISPR-Cas9, a powerful gene editing tool, to transposons, or "jumping genes," which carry RNA-guided DNA nucleases. By studying the movement of transposons in bacteria, scientists discovered that the DNA-cutting scissors prevent the extinction of transposons by guiding a copy of the transposon back to its original location after cutting the DNA. This "cut and copy" strategy allows transposons to proliferate and spread. The findings suggest that there may be other systems similar to CRISPR-Cas9 waiting to be discovered, which could be used for genome engineering in human cells.

via Phys.org|
#crispr#gene-editing#genome-engineering
science-and-technology2 years ago

"Discovery: Fanzor, a Programmable RNA-Guided Endonuclease, Unveils Animal's CRISPR-Like System for Editing Human Genome"

Researchers have discovered a new class of RNA-guided endonuclease called Fanzor, which is found in eukaryotes. Fanzor has the ability to cleave DNA and can be reprogrammed for genome engineering applications in humans. The structure of Fanzor has been resolved, revealing its similarity to other RNA-guided systems like Cas12. This discovery demonstrates that RNA-guided endonucleases are present in all three domains of life.

via Nature.com|
#crispr-cas#eukaryotes#fanzor