"pAblo·pCasso: Revolutionizing CRISPR for Advanced Genome Engineering"

Researchers at The Novo Nordisk Foundation Center for Biosustainability have developed a new CRISPR-Cas toolkit called "pAblo·pCasso," which enables precise and reversible DNA edits within Gram-negative bacteria, expanding the range of genome sites available for base-editing and accelerating the development of bacteria for various bioproduction applications. This technology overcomes limitations of traditional CRISPR systems by not requiring specific PAM sequences and offering reversible editing capability, revolutionizing bacterial engineering and genetic manipulation.