3D Organization of Active and Silenced E. coli Genome

The study uses ultra-high-resolution Micro-C mapping to reveal complex 3D structures in the E. coli genome, including transcription-dependent domains called OPCIDs and repressive structures called CHINs and CHIDs, which are primarily assembled on horizontally transferred genes and regulated by nucleoid-associated proteins like H-NS. These structures influence gene expression and genome stability, with H-NS playing a central role in their formation and maintenance.