Enhancing Genome Editing with the NICER Method

Researchers in Japan have developed a new gene editing technique called NICER, which significantly reduces unintended DNA mutations compared to the conventional CRISPR/Cas9 method. NICER involves creating multiple small cuts in single DNA strands using an enzyme called a nickase, which induces single-strand breaks that are typically repaired without causing mutations. The technique has shown promising results in correcting heterozygous mutations in cells and restoring the expression of disease-causing genes. NICER may provide a safer alternative for treating genetic diseases caused by mutations.