Advanced Prime Editors Achieve Minimal Genomic Errors

Researchers engineered prime editors by modifying Cas9 to relax nick positioning, which promotes degradation of competing DNA strands, significantly reducing insertion and deletion errors while maintaining high editing efficiency across various loci and cell types, including human and mouse cells. These advanced editors, such as the precise prime editor (pPE), extra-precise prime editor (xPE), and very-precise prime editor (vPE), demonstrate improved fidelity and potential for safer, more accurate genome editing applications.