Tag

Two Photon Microscopy

All articles tagged with #two photon microscopy

"Fluoropolymer Nanosheet Enables Large-Scale In Vivo Mouse Brain Imaging"

Originally Published 1 year ago — by Nature.com

A new method utilizing a biocompatible nanosheet incorporated into light-curable resin (NIRE method) has been proposed to construct large cranial windows over the cortex and cerebellum suitable for long-term imaging in awake mice. The NIRE method produced windows that maintained transparency for over 5 months and suppressed motion artifacts, enabling the visualization of neural structures and intracellular Ca2+ concentration changes at various scales, from populations of over a thousand neurons to single spines, in living mouse brain. This method allows for multi-scale in vivo imaging of neuronal morphology and intracellular calcium in awake mice, demonstrating its potential for studying brain function and pathology.

science science-and-technology cranial-window in-vivo-imaging mouse-brain neuroscience science-and-technology two-photon-microscopy

"Neural Rhythms: Unveiling the Brain's Orderly Patterns"

Originally Published 2 years ago — by Nature.com

Researchers conducted experiments on mice to investigate minute-scale oscillatory sequences in the medial entorhinal cortex (MEC). They performed surgeries to implant microprisms or glass windows for optical access to the MEC and adjacent areas. Two-photon calcium imaging and electrophysiological data were collected, and Neuropixels probes were used for recording neural signals. The mice were trained to run on a wheel under minimal sensory stimulation conditions. The study provides insights into the neural activity patterns in the MEC and their potential role in spatial navigation and memory.

science neuroscience calcium-imaging electrophysiology medial-entorhinal-cortex neuropixels-probe neuroscience two-photon-microscopy