Efficient non-transgenic editing of perennial plants using co-editing strategy
Researchers have developed a co-editing strategy for transgene-free genome editing in vegetatively propagated and perennial plant species in the T0 generation. By utilizing CRISPR/Cas9 technology and transient expression of DNA or RNA, the researchers achieved efficient and precise genome editing in various plant species, including wheat, maize, rice, citrus, tomato, potato, and apple. This approach offers a promising method for generating genetically stable and transgene-free edited plants, with potential applications in agriculture and crop improvement.