3D Lipid Mapping Uncovers Lipid Sorting in Early Endosomes
A new Lipid-CLEM workflow combines photo-crosslinkable lipid probes with correlative light and electron microscopy to visualize lipid organization in membranes in 3D, revealing that sphingomyelin concentrates in small endosomal vesicles while avoiding tubular domains, indicating active lipid sorting in early endosomes and potential implications for membrane-related diseases.